Prepare the setups according to how you schedule the activities. The effect of temperature and whether a seed has broken dormancy are quantified and graphed. Great as a field activity to study biochemical oxygen demand bod, respiration rate and primary productivity. Videos anatomy and physiology ap biology ap chemistry. Pearson, as an active contributor to the biology learning community, is pleased to provide free access to the classic edition of the biology place to all educators and their students. Dissolved oxygen in an aquatic environment, oxygen must be in a solution in a free state o 2 before it is available for use by organisms bioavailable. I compared it to a chart of 100 percent oxygen solubility at different temperatures, and in all cases for the chart values at 100 percent, the nomograph reads. Visit ap central at for details about the ap course audit, coursespecific curricular requirements, and how to submit your syllabus for ap course audit authorization.
Choose from 500 different sets of exam ap biology chemistry life campbell flashcards on quizlet. Ap biology lab cellular respiration ap bio lab 6 cellular. As the advanced placement program continues to experience tremendous growth in the twentyfirst. How can the rate of cellular respiration be measured. In this classic water quality testing lab, the relationship between the amount of dissolved oxygen in water and water quality are explored and quantified using the winkler method. The 1station kit includes materials for 1 group of 4 students.
Terrestrial environments hold over 95% more oxygen than aquatic environments. He explains how you can use the pglo plasmid to produce glowing e. Wards offers several options with prenumbered pages for instance, item. I found the discussion of dissolved oxygen in one of my old limnology books to be quite helpful.
In water, the oxygen concentration is much smaller than that. Temperature and dissolved oxygen the advanced placement biology laboratory manual recommends that each student group determine do of water samples at three different temperatures. Rate of cellular respiration davids academic portfolio. If you teach ap, ib, or college biology, you should also consider. Permission granted to make unlimited copies for use in any one school building.
The test results indicated that after a 24hour time period, there was a difference in the primary productivity relative the amount of light tested. The dissolved oxygen probe is great for biology, chemistry, ecology, or integrated science courses. The instructor information section included for each experiment contains tips for. Teachers can modify these labs to make them inquiry based in support of the revised ap biology course. Choose from our kits, follow a college board lab, or design your own with our wide variety of equipment and supplies. The new teacher manual is available as a free pdf on our website. It goes on for several pages so i am only paraphrasing. Dissolved oxygen and primary aquatic productivity laboratory 12 introduction dissolved oxygen levels are an extremely important factor in determining the quality of an aquatic environment.
This lab is a fitting climax to the series of 12 aprecommended biology labs. Preparing for the lab questions on the ap biology exam. Dissolved oxygenclassic lab kit for ap biology, 3 groups. For example, if you follow the schedule suggested in time requirements, above, on lab day 1 students will need all the materials listed for exercise 2a plus 15 ml of h 2o 2 in a 60ml cup labeled h 2 o 2. Ap biology investigative labs ap central the college board. He then describes how you can use restriction enzymes and the process of gel.
The first is the germinated pea which is guaranteed to respire since the peas are growing which means the cells in the peas are dividing therefore undergoing respiration. In the dissolved oxygen classic lab kit for ap biology, the relationship between the amount of do in water and water quality are explored and quantified using the winkler method. Ap biology lab manual for teachers supplement technology integration use of gas pressure probeware provides a different means of running lab 2 a more rapidly, b without the necessity of using caustic sulfuric acid or messy permanganate stain and c without timing problems involved in stopping the reaction at specific points. A b 2 would a cell placed into a hypertonic solution shrink, swell, or stay the same. Introductionthe main focus of the lab was to measure and record the respiration of peas, both germinated and nongerminated, and the effect of.
Analysis of dissolved oxygen lab have faith in ap biology. Biology with vernier is the foundational biology book for high school and college courses. Purpose of this lab the purpose of this lab was to determine the rate of cellular respiration in germinating peas by measuring the consumption of oxygen at various temperatures. Dissolved oxygen is necessary for the metabolic processes of almost every organism. The 8station kit includes enough materials for a class of 32 students working in groups of 4. Chemicals for the winkler technique are provided in groupsized increments.
In part 2, you will measure the water potential in plant cells by measuring the percent. The oxygen produced from the subsequent reaction will become trapped in the disc, thus giving it buoyancy. The time measured from the moment the disc touches the substrate until it reaches the surface of the solution is a measure of the rate of the enzyme activity. Fish and many other aquatic organisms have evolved gills to take up the dissolved oxygen from water.
The advanced placement ap program is a registered trademark of the college entrance examination board. He starts by discussing the process of transformation. It can be used to perform a wide variety of experiments to. Paul andersen explains the two major portions of the molecular biology lab in ap biology. Ap biology lab unit 2 diffusion and osmosis lab purposeobjectives how do cell membranes help regulate internal cellular makeup. By measuring the amount of glucose consumed by measuring the amount of oxygen consumed. Expected results and analysis edit from only having glucose and starch within the dialysis bag, the glucose diffuses and there is a presence of glucose inside the cup that was initially distilled water and the absence of starch. Ap biology lab manual for teachers revised edition by college board author isbn.
An inquirybased approach student manual 2012, which is also available in this store. Dissolved oxygen and aquatic primary productivity kit. Feel free to download a copy of the others if you misplaced any part of your lab manual. Would you expect the dissolved oxygen levels in water sampled from a lake at 7am to be higher or lower than the dissolved oxygen levels in water sampled at 5pm. Ap sample lab 12 dissolved oxygen biology junction. Prepare your students for medical and lab tech careers with carolina s wide range of equipment, kits and models. Mar 14, 2012 paul andersen explains the two major portions of the molecular biology lab in ap biology. Learn exam ap biology chemistry life campbell with free interactive flashcards. Dissolved oxygenclassic lab kit for ap biology, 8 groups. And remember, the oxygen is an indirect measurement of the sugars produced in photosynthesis and lost in respiration. Remove the do probe from the storage bottle and carefully insert it into the culture.
Organisms share many conserved core processes and features that evolved and are. Stay connected to your students with prezi video, now in microsoft teams. To further investigate the rate of cellular respiration in mung bean seeds, there are many future experiments that could be conducted to test the various conditions under which living organisms undergo cellular respiration. A composition book with bound pages is highly recommended. Introductionthe main focus of the lab was to measure and record the respiration of peas, both germinated and nongerminated, and the effect of temperature on the peas. You can save time by assigning each group one temperature of water for do determination and taking class averages. The silver ring on the probe should be immersed in the culture, but the probe should not be touching the bottom of the beaker. Ap bio lab 6 cellular respiration objectives measure oxygen consumption using a respirometer. The concepts align with the enduring understandings and learning objectives from the ap biology curriculum framework, as indicated below. Editorial inquiries concerning this book should be directed to the college board. Learn questions answers ap biology packet with free interactive flashcards.
Their experiment described now in many introductory college textbooks and web sites tested. The only disappointment has been the dissolved oxygen probe. Ap biology cellular respiration lab by katie alvarez on prezi. Diffusion and osmosis 1 list the 2 components affecting the water potential of a system. Prepare your students for medical and lab tech careers with carolina s wide range of.
While i was recently going over the dissolved oxygen data from lab 12, i realized that the nomograph in edition d page 140 of the ap lab manual is very poor and inaccurate. In part 1, you investigate the relationship between the volume and surface area of a model cell and the rate of diffusion. Ap biology lab manual for students revised 2001 on. Andersen demonstrates the azidewinkler method of dissolved oxygen analysis. The silver ring on the probe should be immersed in the culture, but the probe should not. Organisms share many conserved core processes and features that evolved and are widely distributed among organisms today.
We have kits for new and traditional ap biology labs. As we work to adjust to the current reality, make sure to check out these dedicated covid19 resources. Choose from 500 different sets of questions answers ap biology packet flashcards on quizlet. Measure the respiration rate ml o 2 mingram of acheta domesticu s common cricket and tenebrio molitor larvae mealworms at two different temperatures. Using the following diagram, describe the effect that each of the factors has on availability of oxygen and. Ap biology lab cell respiration this investigation uses respirometry techniques to calculate the rate of oxygen consumption cellular respiration in germinating pea seeds. This ap lab attempts to demonstrate the process of osmosis using dialysis bags. After conducting this lab it was determined that primary productivity can indeed be measured using dissolved oxygen levels. It applies knowledge gained in several of the other labs, especially lab 4 on photosynthesis and lab 5 on cellular respiration. These measure relative volume changes in pressure as oxygen is consumed by germinating plant seeds. Measure the amount of glucose oxidized per hour by acheta domesticu s common cricket and tenebrio molitor larvae mealworms at. This barcode number lets you verify that youre getting exactly the right version or edition of a book. Restate the following hypothesis in an ifthenbecause statement.
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